Electrophoresis of ribonucleoproteins reveals an ordered assembly pathway of yeast splicing complexes.
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Human step II splicing factor hSlu7 functions in restructuring the spliceosome between the catalytic steps of splicingIdentification, purification, and biochemical characterization of U2 small nuclear ribonucleoprotein auxiliary factorProteomic analysis identifies a new complex required for nuclear pre-mRNA retention and splicingIdentification and characterization of human genes encoding Hprp3p and Hprp4p, interacting components of the spliceosomeThe HeLa 200 kDa U5 snRNP-specific protein and its homologue in Saccharomyces cerevisiae are members of the DEXH-box protein family of putative RNA helicasesIdentification of an RNA-dependent ATPase activity in mammalian U5 snRNPsThe molecular characterization of PRP6 and PRP9 yeast genes reveals a new cysteine/histidine motif common to several splicing factorsSaccharomyces cerevisiae U1 small nuclear RNA secondary structure contains both universal and yeast-specific domainsEvidence for a Prp24 binding site in U6 snRNA and in a putative intermediate in the annealing of U6 and U4 snRNAsU4 small nuclear RNA dissociates from a yeast spliceosome and does not participate in the subsequent splicing reactionA novel set of spliceosome-associated proteins and the essential splicing factor PSF bind stably to pre-mRNA prior to catalytic step II of the splicing reactionPurification of splicing factor SF1, a heat-stable protein that functions in the assembly of a presplicing complexThe spliceosome assembly pathway in mammalian extracts20S small nuclear ribonucleoprotein U5 shows a surprisingly complex protein compositionEarly commitment of yeast pre-mRNA to the spliceosome pathwayPRP28, a 'DEAD-box' protein, is required for the first step of mRNA splicing in vitroU5 small nuclear ribonucleoprotein: RNA structure analysis and ATP-dependent interaction with U4/U6A U5 small nuclear ribonucleoprotein particle protein involved only in the second step of pre-mRNA splicing in Saccharomyces cerevisiae.Elevated levels of a U4/U6.U5 snRNP-associated protein, Spp381p, rescue a mutant defective in spliceosome maturation.Yeast pre-mRNA splicing requires a pair of U1 snRNP-associated tetratricopeptide repeat proteins.Prp31p promotes the association of the U4/U6 x U5 tri-snRNP with prespliceosomes to form spliceosomes in Saccharomyces cerevisiae.The splicing factor PRP2, a putative RNA helicase, interacts directly with pre-mRNASpliceosome activation by PRP2 ATPase prior to the first transesterification reaction of pre-mRNA splicingThe yeast PRP6 gene encodes a U4/U6 small nuclear ribonucleoprotein particle (snRNP) protein, and the PRP9 gene encodes a protein required for U2 snRNP bindingTransient interaction of BBP/ScSF1 and Mud2 with the splicing machinery affects the kinetics of spliceosome assembly.The yeast homolog of the U1 snRNP protein 70K is encoded by the SNP1 gene.Yeast ortholog of the Drosophila crooked neck protein promotes spliceosome assembly through stable U4/U6.U5 snRNP addition.Interactions of the yeast SF3b splicing factor.Progression through the spliceosome cycle requires Prp38p function for U4/U6 snRNA dissociationThe biochemical defects of prp4-1 and prp6-1 yeast splicing mutants reveal that the PRP6 protein is required for the accumulation of the [U4/U6.U5] tri-snRNP.Identification of Prp40, a novel essential yeast splicing factor associated with the U1 small nuclear ribonucleoprotein particleCef1p is a component of the Prp19p-associated complex and essential for pre-mRNA splicing.Interactions of PRP2 protein with pre-mRNA splicing complexes in Saccharomyces cerevisiae.Rds3p is required for stable U2 snRNP recruitment to the splicing apparatus.Functional association of essential splicing factor(s) with PRP19 in a protein complex.Functional and physical interactions between components of the Prp19p-associated complexThe final stages of spliceosome maturation require Spp2p that can interact with the DEAH box protein Prp2p and promote step 1 of splicing.Snt309p, a component of the Prp19p-associated complex that interacts with Prp19p and associates with the spliceosome simultaneously with or immediately after dissociation of U4 in the same manner as Prp19p.The Clf1p splicing factor promotes spliceosome assembly through N-terminal tetratricopeptide repeat contacts.Yeast U1 snRNP-pre-mRNA complex formation without U1snRNA-pre-mRNA base pairing.
P2860
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P2860
Electrophoresis of ribonucleoproteins reveals an ordered assembly pathway of yeast splicing complexes.
description
1986 nî lūn-bûn
@nan
1986年の論文
@ja
1986年学术文章
@wuu
1986年学术文章
@zh
1986年学术文章
@zh-cn
1986年学术文章
@zh-hans
1986年学术文章
@zh-my
1986年学术文章
@zh-sg
1986年學術文章
@yue
1986年學術文章
@zh-hant
name
Electrophoresis of ribonucleop ...... y of yeast splicing complexes.
@en
Electrophoresis of ribonucleop ...... y of yeast splicing complexes.
@nl
type
label
Electrophoresis of ribonucleop ...... y of yeast splicing complexes.
@en
Electrophoresis of ribonucleop ...... y of yeast splicing complexes.
@nl
prefLabel
Electrophoresis of ribonucleop ...... y of yeast splicing complexes.
@en
Electrophoresis of ribonucleop ...... y of yeast splicing complexes.
@nl
P2093
P356
P1433
P1476
Electrophoresis of ribonucleop ...... y of yeast splicing complexes.
@en
P2093
P2888
P304
P356
10.1038/324341A0
P407
P577
1986-11-01T00:00:00Z
P6179
1014362908