Thiophosphate interference experiments locate phosphates important for the hammerhead RNA self-cleavage reaction.
about
Defining the chemical groups essential for Tetrahymena group I intron function by nucleotide analog interference mappingDissecting RNA folding by nucleotide analog interference mapping (NAIM)Redesigned and chemically-modified hammerhead ribozymes with improved activity and serum stabilityBackbone and nucleobase contacts to glucosamine-6-phosphate in the glmS ribozymeRecent advances in the elucidation of the mechanisms of action of ribozymesHammerhead redux: does the new structure fit the old biochemical data?Structural basis for recognition of Co2+ by RNA aptamers.The hammerhead ribozyme: structure, catalysis, and gene regulationA further investigation and reappraisal of the thio effect in the cleavage reaction catalyzed by a hammerhead ribozyme.Identification and characterization of a novel high affinity metal-binding site in the hammerhead ribozymeThiophilic metal ion rescue of phosphorothioate interference within the Tetrahymena ribozyme P4-P6 domain.Differences in the phosphate oxygen requirements for self-cleavage by the extended and prototypical hammerhead formsIdentification of phosphate oxygens that are important for self-cleavage activity of the HDV ribozyme by phosphorothioate substitution interference analysis.Nuclease-resistant chimeric ribozymes containing deoxyribonucleotides and phosphorothioate linkages.RNA template-directed RNA synthesis by T7 RNA polymeraseThiophosphates in yeast U6 snRNA specifically affect pre-mRNA splicing in vitro.Enhancement of ribozyme catalytic activity by a contiguous oligodeoxynucleotide (facilitator) and by 2'-O-methylation.A phosphorothioate at the 3' splice-site inhibits the second splicing step in a group I intron.Observations on catalysis by hammerhead ribozymes are consistent with a two-divalent-metal-ion mechanism.Ribozyme catalysis: not different, just worse.Substrate-assisted catalysis in the cleavage of DNA by the EcoRI and EcoRV restriction enzymesBridging the gap between theory and experiment to derive a detailed understanding of hammerhead ribozyme catalysis.RNA structure and NMR spectroscopy.In vitro selection for altered divalent metal specificity in the RNase P RNA.Efficient substrate cleavage catalyzed by hammerhead ribozymes derivatized with selenium for X-ray crystallography.Determinant nucleotides of yeast tRNA(Asp) interact directly with aspartyl-tRNA synthetase.Importance of specific purine amino and hydroxyl groups for efficient cleavage by a hammerhead ribozyme.The roles of the conserved pyrimidine bases in hammerhead ribozyme catalysis: evidence for a magnesium ion-binding site.Ion-induced folding of the hammerhead ribozyme: a fluorescence resonance energy transfer study.Multiple metal-binding cores are required for metalloregulation by M-box riboswitch RNAsMultiple conformational states of the hammerhead ribozyme, broad time range of relaxation and topology of dynamicsInteraction of mRNA with the Escherichia coli ribosome: accessibility of phosphorothioate-containing mRNA bound to ribosomes for iodine cleavage.The efficiency of a cis-cleaving ribozyme in an mRNA coding region is influenced by the translating ribosome in vivoSingle substitutions of phosphorothioates in the HDV ribozyme G73 define regions necessary for optimal self-cleaving activity.Recent developments in the hammerhead ribozyme field.Cleavage properties of an oligonucleotide containing a bridged internucleotide 5'-phosphorothioate RNA linkageModification interference approach to detect ribose moieties important for the optimal activity of a ribozyme.Phosphorothioate substitution identifies phosphate groups important for pre-mRNA splicingExtensive phosphorothioate substitution yields highly active and nuclease-resistant hairpin ribozymes.Cryoenzymology of the hammerhead ribozyme.
P2860
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P2860
Thiophosphate interference experiments locate phosphates important for the hammerhead RNA self-cleavage reaction.
description
1990 nî lūn-bûn
@nan
1990年の論文
@ja
1990年論文
@yue
1990年論文
@zh-hant
1990年論文
@zh-hk
1990年論文
@zh-mo
1990年論文
@zh-tw
1990年论文
@wuu
1990年论文
@zh
1990年论文
@zh-cn
name
Thiophosphate interference exp ...... ad RNA self-cleavage reaction.
@en
type
label
Thiophosphate interference exp ...... ad RNA self-cleavage reaction.
@en
prefLabel
Thiophosphate interference exp ...... ad RNA self-cleavage reaction.
@en
P2860
P356
P1476
Thiophosphate interference exp ...... ad RNA self-cleavage reaction.
@en
P2093
D E Ruffner
O C Uhlenbeck
P2860
P304
P356
10.1093/NAR/18.20.6025
P577
1990-10-01T00:00:00Z