Functional analysis of the U5 snRNA loop 1 in the second catalytic step of yeast pre-mRNA splicing.
about
Cwc2 and its human homologue RBM22 promote an active conformation of the spliceosome catalytic centrePrp8 protein: at the heart of the spliceosomeCharacterization of U6 snRNA-protein interactions.PSF and p54nrb bind a conserved stem in U5 snRNAEvidence for a role of Sky1p-mediated phosphorylation in 3' splice site recognition involving both Prp8 and Prp17/Slu4.Functional interactions between Prp8, Prp18, Slu7, and U5 snRNA during the second step of pre-mRNA splicing.Pseudouridine mapping in the Saccharomyces cerevisiae spliceosomal U small nuclear RNAs (snRNAs) reveals that pseudouridine synthase pus1p exhibits a dual substrate specificity for U2 snRNA and tRNA.The carboxy terminal WD domain of the pre-mRNA splicing factor Prp17p is critical for function.ATP-dependent interaction of yeast U5 snRNA loop 1 with the 5' splice site.RNA structure analysis of human spliceosomes reveals a compact 3D arrangement of snRNAs at the catalytic core.Mutations in U5 snRNA loop 1 influence the splicing of different genes in vivo.Genetic and functional interaction of evolutionarily conserved regions of the Prp18 protein and the U5 snRNA.The RNA splicing factor hSlu7 is required for correct 3' splice-site choice.Conserved loop I of U5 small nuclear RNA is dispensable for both catalytic steps of pre-mRNA splicing in HeLa nuclear extracts.Proximity of the invariant loop of U5 snRNA to the second intron residue during pre-mRNA splicing.Trans-complementation of the second step of pre-mRNA splicing by exogenous 5' exons.Splint ligation of RNA with T4 DNA ligaseAllele-specific genetic interactions between Prp8 and RNA active site residues suggest a function for Prp8 at the catalytic core of the spliceosome.The Prp18 protein stabilizes the interaction of both exons with the U5 snRNA during the second step of pre-mRNA splicingFunctional roles of protein splicing factors.The U1, U2 and U5 snRNAs crosslink to the 5' exon during yeast pre-mRNA splicing.Finding exonic islands in a sea of non-coding sequence: splicing related constraints on protein composition and evolution are common in intron-rich genomes.Splicing of mRNA precursors: the role of RNAs and proteins in catalysis.The mechanism of the second step of pre-mRNA splicing.Group II intron lariat: Structural insights into the spliceosome.Role of Cwc24 in the First Catalytic Step of Splicing and Fidelity of 5' Splice Site Selection.Human GC-AG alternative intron isoforms with weak donor sites show enhanced consensus at acceptor exon positions.Evolution of small nuclear RNAs in S. cerevisiae, C. albicans, and other hemiascomycetous yeasts.The U5 snRNA internal loop 1 is a platform for Brr2, Snu114 and Prp8 protein binding during U5 snRNP assembly.Cwc21p promotes the second step conformation of the spliceosome and modulates 3' splice site selection.Structural and functional characterization of the N terminus of Schizosaccharomyces pombe Cwf10.Functional contacts with a range of splicing proteins suggest a central role for Brr2p in the dynamic control of the order of events in spliceosomes of Saccharomyces cerevisiae.The NineTeen Complex (NTC) and NTC-associated proteins as targets for spliceosomal ATPase action during pre-mRNA splicing.Mutation in the U2 snRNA influences exon interactions of U5 snRNA loop 1 during pre-mRNA splicing.Dissection of Prp8 protein defines multiple interactions with crucial RNA sequences in the catalytic core of the spliceosome.Analysis of synthetic lethality reveals genetic interactions between the GTPase Snu114p and snRNAs in the catalytic core of the Saccharomyces cerevisiae spliceosome.Mutations in the U5 snRNA result in altered splicing of subsets of pre-mRNAs and reduced stability of Prp8.The Prp19-associated complex is required for specifying interactions of U5 and U6 with pre-mRNA during spliceosome activation.Up-Frameshift Protein UPF1 Regulates Neurospora crassa Circadian and Diurnal Growth Rhythms.The recruitment of the U5 snRNP to nascent transcripts requires internal loop 1 of U5 snRNA.
P2860
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P2860
Functional analysis of the U5 snRNA loop 1 in the second catalytic step of yeast pre-mRNA splicing.
description
1998 nî lūn-bûn
@nan
1998 թուականի Յունուարին հրատարակուած գիտական յօդուած
@hyw
1998 թվականի հունվարին հրատարակված գիտական հոդված
@hy
1998年の論文
@ja
1998年論文
@yue
1998年論文
@zh-hant
1998年論文
@zh-hk
1998年論文
@zh-mo
1998年論文
@zh-tw
1998年论文
@wuu
name
Functional analysis of the U5 ...... ep of yeast pre-mRNA splicing.
@ast
Functional analysis of the U5 ...... ep of yeast pre-mRNA splicing.
@en
type
label
Functional analysis of the U5 ...... ep of yeast pre-mRNA splicing.
@ast
Functional analysis of the U5 ...... ep of yeast pre-mRNA splicing.
@en
prefLabel
Functional analysis of the U5 ...... ep of yeast pre-mRNA splicing.
@ast
Functional analysis of the U5 ...... ep of yeast pre-mRNA splicing.
@en
P2860
P356
P1433
P1476
Functional analysis of the U5 ...... ep of yeast pre-mRNA splicing.
@en
P2093
P2860
P304
P356
10.1093/EMBOJ/17.2.565
P407
P577
1998-01-01T00:00:00Z