Deep sequencing of systematic combinatorial libraries reveals β-lactamase sequence constraints at high resolution.
about
Structural insights into N-terminal to C-terminal interactions and implications for thermostability of a (β/α)8-triosephosphate isomerase barrel enzymeRemoval of the Side Chain at the Active-Site Serine by a Glycine Substitution Increases the Stability of a Wide Range of Serine β-Lactamases by Relieving Steric StrainDetection and sequence/structure mapping of biophysical constraints to protein variation in saturated mutational libraries and protein sequence alignments with a dedicated serverA Protocol for Functional Assessment of Whole-Protein Saturation Mutagenesis Libraries Utilizing High-Throughput Sequencing.Molecular Determinants of Mutant Phenotypes, Inferred from Saturation Mutagenesis DataA comprehensive, high-resolution map of a gene's fitness landscapeHigh-throughput mutagenesis reveals functional determinants for DNA targeting by activation-induced deaminase.Coevolutionary Landscape Inference and the Context-Dependence of Mutations in Beta-Lactamase TEM-1.How structural and physicochemical determinants shape sequence constraints in a functional enzymeSystematic Mapping of Protein Mutational Space by Prolonged Drift Reveals the Deleterious Effects of Seemingly Neutral MutationsHigh-Throughput Ligand Discovery Reveals a Sitewise Gradient of Diversity in Broadly Evolved Hydrophilic Fibronectin DomainsDeep Sequencing of Random Mutant Libraries Reveals the Active Site of the Narrow Specificity CphA Metallo-β-Lactamase is Fragile to Mutations.Characterization of the global stabilizing substitution A77V and its role in the evolution of CTX-M β-lactamases.Low-stringency selection of TEM1 for BLIP shows interface plasticity and selection for faster binders.Antibiotics as selectors and accelerators of diversity in the mechanisms of resistance: from the resistome to genetic plasticity in the β-lactamases worldCapturing the mutational landscape of the beta-lactamase TEM-1.Computational tools help improve protein stability but with a solubility tradeoff.The power of multiplexed functional analysis of genetic variants.Mutation effects predicted from sequence co-variation.Deep sequencing methods for protein engineering and design.A comprehensive biophysical description of pairwise epistasis throughout an entire protein domain.Adaptive benefits from small mutation supplies in an antibiotic resistance enzyme.Structural and Mechanistic Basis for Extended-Spectrum Drug-Resistance Mutations in Altering the Specificity of TEM, CTX-M, and KPC β-lactamases.Differential active site requirements for NDM-1 β-lactamase hydrolysis of carbapenem versus penicillin and cephalosporin antibioticsRole of simple descriptors and applicability domain in predicting change in protein thermostabilityMultiplexed assays of variant effects contribute to a growing genotype-phenotype atlas
P2860
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P2860
Deep sequencing of systematic combinatorial libraries reveals β-lactamase sequence constraints at high resolution.
description
2012 nî lūn-bûn
@nan
2012 թուականի Սեպտեմբերին հրատարակուած գիտական յօդուած
@hyw
2012 թվականի սեպտեմբերին հրատարակված գիտական հոդված
@hy
2012年の論文
@ja
2012年論文
@yue
2012年論文
@zh-hant
2012年論文
@zh-hk
2012年論文
@zh-mo
2012年論文
@zh-tw
2012年论文
@wuu
name
Deep sequencing of systematic ...... onstraints at high resolution.
@ast
Deep sequencing of systematic ...... onstraints at high resolution.
@en
Deep sequencing of systematic ...... onstraints at high resolution.
@nl
type
label
Deep sequencing of systematic ...... onstraints at high resolution.
@ast
Deep sequencing of systematic ...... onstraints at high resolution.
@en
Deep sequencing of systematic ...... onstraints at high resolution.
@nl
prefLabel
Deep sequencing of systematic ...... onstraints at high resolution.
@ast
Deep sequencing of systematic ...... onstraints at high resolution.
@en
Deep sequencing of systematic ...... onstraints at high resolution.
@nl
P2093
P2860
P1476
Deep sequencing of systematic ...... onstraints at high resolution.
@en
P2093
Carolyn J Adamski
Erol Bakkalbasi
Kacie Rice
Nicholas G Brown
Richard A Gibbs
Timothy Palzkill
Wanzhi Huang
Zhifeng Deng
P2860
P304
P356
10.1016/J.JMB.2012.09.014
P407
P50
P577
2012-09-25T00:00:00Z