In vitro synthesis of fully functional EmrE, a multidrug transporter, and study of its oligomeric state
about
Investigation of ligand binding to the multidrug resistance protein EmrE by isothermal titration calorimetryX-ray structure of EmrE supports dual topology model.Evidence for assembly of small multidrug resistance proteins by a "two-faced" transmembrane helix.Liposome-mediated cellular delivery of active gp91(phox).Preparative scale production of functional mouse aquaporin 4 using different cell-free expression modes.EmrE dimerization depends on membrane environment.Topologically random insertion of EmrE supports a pathway for evolution of inverted repeats in ion-coupled transportersExpression of neurotransmitter transporters for structural and biochemical studiesAn Escherichia coli-based cell-free system for large-scale production of functional mammalian membrane proteins suitable for X-ray crystallography.Making water-soluble integral membrane proteins in vivo using an amphipathic protein fusion strategy.Parallel topology of genetically fused EmrE homodimers.Solution NMR of membrane proteins in bilayer mimics: small is beautiful, but sometimes bigger is better.Cell-free expression as an emerging technique for the large scale production of integral membrane protein.Optimization of protein expression systems for modern drug discovery.Production of membrane proteins using cell-free expression systems.NMR and EPR studies of membrane transporters.Functional expression and characterisation of membrane transport proteins.Modulation of substrate efflux in bacterial small multidrug resistance proteins by mutations at the dimer interface.In vitro synthesis, tetramerization and single channel characterization of virus-encoded potassium channel Kcv.Efficacy of a potential trivalent vaccine based on Hc fragments of botulinum toxins A, B, and E produced in a cell-free expression system.The assembly motif of a bacterial small multidrug resistance protein.Cell-free complements in vivo expression of the E. coli membrane proteome.Drug efflux by a small multidrug resistance protein is inhibited by a transmembrane peptide.Fluorinated and hemifluorinated surfactants as alternatives to detergents for membrane protein cell-free synthesis.Exploring the role of a unique carboxyl residue in EmrE by mass spectrometry.Production of functional bacteriorhodopsin by an Escherichia coli cell-free protein synthesis system supplemented with steroid detergent and lipidEfflux by small multidrug resistance proteins is inhibited by membrane-interactive helix-stapled peptides.Polymer-based cell-free expression of ligand-binding family B G-protein coupled receptors without detergentsFunctional response of the small multidrug resistance protein EmrE to mutations in transmembrane helix 2.EmrE, a multidrug transporter from Escherichia coli, transports monovalent and divalent substrates with the same stoichiometry.Exploring the binding domain of EmrE, the smallest multidrug transporter.The key residue for substrate transport (Glu14) in the EmrE dimer is asymmetric.Exploiting Leishmania tarentolae cell-free extracts for the synthesis of human solute carriers.On parallel and antiparallel topology of a homodimeric multidrug transporter.Identification of tyrosine residues critical for the function of an ion-coupled multidrug transporter.Emulating membrane protein evolution by rational designFunctional analysis of cell-free-produced human endothelin B receptor reveals transmembrane segment 1 as an essential area for ET-1 binding and homodimer formationSmall Multidrug Resistance Efflux Pumps
P2860
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P2860
In vitro synthesis of fully functional EmrE, a multidrug transporter, and study of its oligomeric state
description
2004 nî lūn-bûn
@nan
2004年の論文
@ja
2004年論文
@yue
2004年論文
@zh-hant
2004年論文
@zh-hk
2004年論文
@zh-mo
2004年論文
@zh-tw
2004年论文
@wuu
2004年论文
@zh
2004年论文
@zh-cn
name
In vitro synthesis of fully fu ...... study of its oligomeric state
@ast
In vitro synthesis of fully fu ...... study of its oligomeric state
@en
type
label
In vitro synthesis of fully fu ...... study of its oligomeric state
@ast
In vitro synthesis of fully fu ...... study of its oligomeric state
@en
prefLabel
In vitro synthesis of fully fu ...... study of its oligomeric state
@ast
In vitro synthesis of fully fu ...... study of its oligomeric state
@en
P2093
P2860
P356
P1476
In vitro synthesis of fully fu ...... study of its oligomeric state
@en
P2093
Sonia Steiner-Mordoch
Tsafi Danieli
Yael Elbaz
P2860
P304
P356
10.1073/PNAS.0306533101
P407
P577
2004-01-30T00:00:00Z