Effects of methotrexate on transfected DNA stability in mammalian cells.
about
Overexpression of human alpha-galactosidase A results in its intracellular aggregation, crystallization in lysosomes, and selective secretionCHO expression of a novel human recombinant IgG1 anti-RhD antibody isolated by phage display.Production of recombinant protein therapeutics in cultivated mammalian cells.Fusion of the Dhfr/Mtx and IR/MAR gene amplification methods produces a rapid and efficient method for stable recombinant protein production.Advances in animal cell recombinant protein production: GS-NS0 expression system.Stability of protein production from recombinant mammalian cells.Engraftment and long-term expression of human fetal hemopoietic stem cells in sheep following transplantation in utero.Ubiquitous Chromatin Opening Elements (UCOEs) effect on transgene position and expression stability in CHO cells following methotrexate (MTX) amplification.Genetic analysis of the clonal stability of Chinese hamster ovary cells for recombinant protein production.It's time to regulate: coping with product-induced nongenetic clonal instability in CHO cell lines via regulated protein expression.Addition of valproic acid to CHO cell fed-batch cultures improves monoclonal antibody titers.Evaluating the influence of selection markers on obtaining selected pools and stable cell lines in human cells.Early prediction of instability of Chinese hamster ovary cell lines expressing recombinant antibodies and antibody-fusion proteins.Promoter methylation and transgene copy numbers predict unstable protein production in recombinant Chinese hamster ovary cell lines.A mechanistic understanding of production instability in CHO cell lines expressing recombinant monoclonal antibodies.Maximizing productivity of CHO cell-based fed-batch culture using chemically defined media conditions and typical manufacturing equipment.Evaluation of stable and highly productive gene amplified CHO cell line based on the location of amplified genes.A study of monoclonal antibody-producing CHO cell lines: what makes a stable high producer?Calcium phosphate transfection generates mammalian recombinant cell lines with higher specific productivity than polyfection.Stable expression of a heterogeneous gene introduced via gene targeting into the HPRT locus of human fibrosarcoma cells.Molecular definition of predictive indicators of stable protein expression in recombinant NS0 myeloma cells.Efficient selection of high-producing subclones during gene amplification of recombinant Chinese hamster ovary cells by flow cytometry and cell sorting.Gene transfer and amplification in CHO cells. Efficient methods for maximizing specific productivity and assessment of genetic consequences.High-level expression of a recombinant antibody from myeloma cells using a glutamine synthetase gene as an amplifiable selectable marker.Relationship of c-myc gene copy number and gene expression: cellular effects of elevated c-myc protein.Simultaneous flow cytometric detection of cellular c-myc protein, incorporated bromodeoxyuridine, and DNA.Identification of transgene integration loci of different highly expressing recombinant CHO cell lines by FISH.Characterization of the stability of recombinant protein production in the GS-NS0 expression system.Comparison of strategies to detect and quantitate uniquely marked cells in intra- and inter-species hemopoietic chimeras.Comparative Assessment on the Expression Level of Recombinant Human Follicle-Stimulating Hormone (FSH) in Serum-Containing Versus Protein-Free Culture Media.Differential stability of proteolytically active and inactive recombinant metalloproteinase in Chinese hamster ovary cells.Effect of cDNA copy number on secretion rate of activated protein C.
P2860
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P2860
Effects of methotrexate on transfected DNA stability in mammalian cells.
description
1990 nî lūn-bûn
@nan
1990年の論文
@ja
1990年論文
@yue
1990年論文
@zh-hant
1990年論文
@zh-hk
1990年論文
@zh-mo
1990年論文
@zh-tw
1990年论文
@wuu
1990年论文
@zh
1990年论文
@zh-cn
name
Effects of methotrexate on transfected DNA stability in mammalian cells.
@ast
Effects of methotrexate on transfected DNA stability in mammalian cells.
@en
type
label
Effects of methotrexate on transfected DNA stability in mammalian cells.
@ast
Effects of methotrexate on transfected DNA stability in mammalian cells.
@en
prefLabel
Effects of methotrexate on transfected DNA stability in mammalian cells.
@ast
Effects of methotrexate on transfected DNA stability in mammalian cells.
@en
P2093
P2860
P356
P1476
Effects of methotrexate on transfected DNA stability in mammalian cells.
@en
P2093
P2860
P304
P356
10.1128/MCB.10.1.401
P407
P577
1990-01-01T00:00:00Z