Cloning of the RNA8 gene of Saccharomyces cerevisiae, detection of the RNA8 protein, and demonstration that it is essential for nuclear pre-mRNA splicing
about
Prp8 protein: at the heart of the spliceosomeThe human Prp8 protein is a component of both U2- and U12-dependent spliceosomesCharacterization of U6 snRNA-protein interactions.A mammalian protein of 220 kDa binds pre-mRNAs in the spliceosome: a potential homologue of the yeast PRP8 proteinThe mammalian analogue of the yeast PRP8 splicing protein is present in the U4/5/6 small nuclear ribonucleoprotein particle and the spliceosomeA U5 small nuclear ribonucleoprotein particle protein involved only in the second step of pre-mRNA splicing in Saccharomyces cerevisiae.Assembly of Snu114 into U5 snRNP requires Prp8 and a functional GTPase domain.A comprehensive biochemical and genetic analysis of the yeast U1 snRNP reveals five novel proteins.Functional interactions between Prp8, Prp18, Slu7, and U5 snRNA during the second step of pre-mRNA splicing.prp8 mutations that cause human retinitis pigmentosa lead to a U5 snRNP maturation defect in yeastInteractions of PRP2 protein with pre-mRNA splicing complexes in Saccharomyces cerevisiae.Domain analysis of human U5 RNA. Cap trimethylation, protein binding, and spliceosome assemblyA novel 40S multi-snRNP complex isolated from rat liver nucleiTowards the prediction of essential genes by integration of network topology, cellular localization and biological process information.PRP5: a helicase-like protein required for mRNA splicing in yeastConserved loop I of U5 small nuclear RNA is dispensable for both catalytic steps of pre-mRNA splicing in HeLa nuclear extracts.Genetic depletion indicates a late role for U5 snRNP during in vitro spliceosome assembly.Functional roles of protein splicing factors.PRP18, a protein required for the second reaction in pre-mRNA splicing.PRP4 (RNA4) from Saccharomyces cerevisiae: its gene product is associated with the U4/U6 small nuclear ribonucleoprotein particle.Affinity purification of spliceosomes reveals that the precursor RNA processing protein PRP8, a protein in the U5 small nuclear ribonucleoprotein particle, is a component of yeast spliceosomes.RNA biology in a test tube--an overview of in vitro systems/assays.Extensive interactions of PRP8 protein with the 5' and 3' splice sites during splicing suggest a role in stabilization of exon alignment by U5 snRNA.Nuclear pre-mRNA splicing in yeast.A functional homologue of the RNA1 gene product in Schizosaccharomyces pombe: purification, biochemical characterization, and identification of a leucine-rich repeat motif.The essential yeast Tcp1 protein affects actin and microtubules.Interaction of the yeast splicing factor PRP8 with substrate RNA during both steps of splicing.Antisense probes containing 2-aminoadenosine allow efficient depletion of U5 snRNP from HeLa splicing extracts.The yeast PRP8 protein interacts directly with pre-mRNA.A dominant negative mutation in the conserved RNA helicase motif 'SAT' causes splicing factor PRP2 to stall in spliceosomes.Pre-mRNA splicing mutants of Schizosaccharomyces pombe.Roles of PRP8 protein in the assembly of splicing complexes.Functional contacts with a range of splicing proteins suggest a central role for Brr2p in the dynamic control of the order of events in spliceosomes of Saccharomyces cerevisiae.Trans mRNA splicing in trypanosomes: cloning and analysis of a PRP8-homologous gene from Trypanosoma brucei provides evidence for a U5-analogous RNPMechanistic insights into precursor messenger RNA splicing by the spliceosome.Extraordinary sequence conservation of the PRP8 splicing factor.
P2860
Q24537373-0074C70D-F9CB-40D1-B8FE-98B7E45BCBE1Q24539395-7170ABE4-643D-47FD-9273-4E1020912704Q24539483-8D49AA36-F9DD-4840-9279-85633F618F29Q24558707-676559DA-C03D-454C-BF7E-D79C78887C12Q24603409-19B9F68D-C0AE-4F0E-B815-0F8E73C72004Q27930011-010B0702-E504-44E3-991C-6B241FC280BDQ27932833-8BAD12F0-E88C-4F64-B124-56375C3E8356Q27934040-F69E0218-EAED-4571-B9EF-22A77844644DQ27935202-32292F87-B0C4-49DE-9591-E195BCC889D1Q27935241-DAF1B17C-83A9-42DC-804E-974E5E484A67Q27938316-8F96B8E1-42DF-4BD3-8350-3C5BABD17FB2Q28284343-7639CE93-BF64-4B3F-9F73-9F1C08EE8FF6Q28569746-9CABB352-4F95-4D9D-B48C-5660E394237AQ33503874-098B7DE4-612B-4D00-8B56-59527BFA8737Q33612385-B8E9C054-1547-4F02-B349-7B4F5C507C04Q33957723-760ADC6A-DEA5-4DC8-8300-FB67E508CED9Q35772696-113BA406-30B1-48A9-B42E-CF380EF78616Q36083004-5660ADBC-0B2B-457F-87BD-A42F4029B91CQ36714216-161D3518-FE9A-4D76-8F0E-801EBE7D48BDQ36759940-6EFA0DD4-5018-47DE-9D9D-FFDDF4F97CF5Q37720817-76B63317-2307-477A-A3C0-5E038949CC1BQ37996957-9C78FEF2-7926-41E3-9C46-FEDF12EF0E47Q38294598-7724A67A-EAB8-4A26-9AA9-E81B7493E7FCQ38762332-8375648E-3ECE-437B-8924-6C5974BFC079Q40365606-5AAC3033-5833-48B7-8E9E-9E176EF52353Q40366371-643AA65D-2136-43CD-9449-F911AFA99B65Q40392773-34994EBE-026F-4258-ACFF-29F35C8F6A49Q40504521-94105855-41F8-4D30-BD44-12F497D16865Q40507815-D3DF7E57-BB89-487E-B39E-AB15D6C3B864Q40790922-FB94BAFE-CF70-4B7C-956A-F1FC96A40A43Q40817226-F7BCA8C6-808B-4DD5-84DC-E0C99B0CEC3AQ41533943-3AF5DDE3-6903-4E1A-914B-A00188FDB123Q41857700-62F82783-6809-4CA2-967C-B90BEA95AA2BQ42625366-FE7F1F95-1767-4615-9C63-0C095DFE318AQ47738832-05F4E573-F8AE-4132-9872-35538F7FA551Q48074467-4CA10FA0-3C79-4797-B996-D2F664977E86
P2860
Cloning of the RNA8 gene of Saccharomyces cerevisiae, detection of the RNA8 protein, and demonstration that it is essential for nuclear pre-mRNA splicing
description
1988 nî lūn-bûn
@nan
1988年の論文
@ja
1988年論文
@yue
1988年論文
@zh-hant
1988年論文
@zh-hk
1988年論文
@zh-mo
1988年論文
@zh-tw
1988年论文
@wuu
1988年论文
@zh
1988年论文
@zh-cn
name
Cloning of the RNA8 gene of Sa ...... for nuclear pre-mRNA splicing
@en
type
label
Cloning of the RNA8 gene of Sa ...... for nuclear pre-mRNA splicing
@en
prefLabel
Cloning of the RNA8 gene of Sa ...... for nuclear pre-mRNA splicing
@en
P2093
P2860
P356
P1476
Cloning of the RNA8 gene of Sa ...... for nuclear pre-mRNA splicing
@en
P2093
P2860
P304
P356
10.1128/MCB.8.3.1067
P407
P577
1988-03-01T00:00:00Z