Suppressors of a cold-sensitive mutation in yeast U4 RNA define five domains in the splicing factor Prp8 that influence spliceosome activation.
about
Prp8 protein: at the heart of the spliceosomePrp8p dissection reveals domain structure and protein interaction sites.Structure and interactions of the first three RNA recognition motifs of splicing factor prp24The architecture of the spliceosomal U4/U6.U5 tri-snRNP.Crystal structure of the C-terminal domain of splicing factor Prp8 carrying retinitis pigmentosa mutantsCrystal structure of the -finger domain of Prp8 reveals analogy to ribosomal proteinsStructure and function of an RNase H domain at the heart of the spliceosomeCrystal structure of Prp8 reveals active site cavity of the spliceosomeStructural basis for dual roles of Aar2p in U5 snRNP assemblyA conformational switch in PRP8 mediates metal ion coordination that promotes pre-mRNA exon ligationStructural Basis of Brr2-Prp8 Interactions and Implications for U5 snRNP Biogenesis and the Spliceosome Active SiteCore structure of the U6 small nuclear ribonucleoprotein at 1.7-Å resolutionEvidence for a role of Sky1p-mediated phosphorylation in 3' splice site recognition involving both Prp8 and Prp17/Slu4.Assembly of Snu114 into U5 snRNP requires Prp8 and a functional GTPase domain.Comprehensive in vivo RNA-binding site analyses reveal a role of Prp8 in spliceosomal assembly.Inhibition of a spliceosome turnover pathway suppresses splicing defects.Genetic analysis reveals a role for the C terminus of the Saccharomyces cerevisiae GTPase Snu114 during spliceosome activationPrp8 retinitis pigmentosa mutants cause defects in the transition between the catalytic steps of splicing.Characterization of U4 and U6 interactions with the 5' splice site using a S. cerevisiae in vitro trans-splicing systemPrp8, the pivotal protein of the spliceosomal catalytic center, evolved from a retroelement-encoded reverse transcriptaseDistinct domains of splicing factor Prp8 mediate different aspects of spliceosome activationProximity of the invariant loop of U5 snRNA to the second intron residue during pre-mRNA splicing.Ubiquitin binding by a variant Jab1/MPN domain in the essential pre-mRNA splicing factor Prp8p.Spliceosome assembly in the absence of stable U4/U6 RNA pairing.FgPrp4 Kinase Is Important for Spliceosome B-Complex Activation and Splicing Efficiency in Fusarium graminearum.A dynamic bulge in the U6 RNA internal stem-loop functions in spliceosome assembly and activation.Spliceosome activation: U4 is the path, stem I is the goal, and Prp8 is the keeper. Let's cheer for the ATPase Brr2!Exo70, a subunit of the exocyst complex, interacts with SNEV(hPrp19/hPso4) and is involved in pre-mRNA splicingSubstrate-assisted mechanism of RNP disruption by the spliceosomal Brr2 RNA helicase.ATP-dependent unwinding of U4/U6 snRNAs by the Brr2 helicase requires the C terminus of Prp8.Physical and genetic interactions of yeast Cwc21p, an ortholog of human SRm300/SRRM2, suggest a role at the catalytic center of the spliceosome.The spliceosomal proteome: at the heart of the largest cellular ribonucleoprotein machine.Structural analyses of the pre-mRNA splicing machineryStructural studies of the spliceosome: zooming into the heart of the machine.Novel regulatory principles of the spliceosomal Brr2 RNA helicase and links to retinal disease in humans.Cryo-EM structure of the yeast U4/U6.U5 tri-snRNP at 3.7 Å resolution.Structural toggle in the RNaseH domain of Prp8 helps balance splicing fidelity and catalytic efficiency.Genetics and biochemistry remain essential in the structural era of the spliceosome.An unanticipated early function of DEAD-box ATPase Prp28 during commitment to splicing is modulated by U5 snRNP protein Prp8.The Prp8 RNase H-like domain inhibits Brr2-mediated U4/U6 snRNA unwinding by blocking Brr2 loading onto the U4 snRNA.
P2860
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P2860
Suppressors of a cold-sensitive mutation in yeast U4 RNA define five domains in the splicing factor Prp8 that influence spliceosome activation.
description
2000 nî lūn-bûn
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2000 թուականի Օգոստոսին հրատարակուած գիտական յօդուած
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2000 թվականի օգոստոսին հրատարակված գիտական հոդված
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Suppressors of a cold-sensitiv ...... luence spliceosome activation.
@ast
Suppressors of a cold-sensitiv ...... luence spliceosome activation.
@en
Suppressors of a cold-sensitiv ...... luence spliceosome activation.
@nl
type
label
Suppressors of a cold-sensitiv ...... luence spliceosome activation.
@ast
Suppressors of a cold-sensitiv ...... luence spliceosome activation.
@en
Suppressors of a cold-sensitiv ...... luence spliceosome activation.
@nl
prefLabel
Suppressors of a cold-sensitiv ...... luence spliceosome activation.
@ast
Suppressors of a cold-sensitiv ...... luence spliceosome activation.
@en
Suppressors of a cold-sensitiv ...... luence spliceosome activation.
@nl
P2860
P1433
P1476
Suppressors of a cold-sensitiv ...... luence spliceosome activation.
@en
P2093
P2860
P304
P407
P577
2000-08-01T00:00:00Z